CV-1 Nuclei Production
CV-1 cells are grown in cell-factories under GLP conditions in our facility in Mons, Belgium.

Nuclei are prepared by low speed centrifugation, rinsed with phosphate buffer saline. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Cytoplasm is then snap frozen in liquid nitrogen and stored at -85°C.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).

Quality Control
Cultures are screened for the presence of bacteria, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.

HeLa Cytoplasmic Extracts Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Ipracell, provider of high quality HeLa Nuclear Extract (HNE) expands its activities by making available the cytoplasm prepared from exponentially growing HeLa cells (HeLa Cytoplasmic Extract: HCE).
HCE is a rich source of:

• complexes (40S, 60S ribosomal subunits and 80S ribosomes etc…)
• Factors involved in translation (eIFs’, IRES- and PolyA binding proteins).
• Factors which play a role on different parts of the mRNA molecule (5′- and 3′ UTRs’).

It can be used to study the regulation of muRNA processing, mRNA polymorphism etc…
Preliminary data suggest that HCE compares favorably with the rabbit reticulocyte lysate for cell-free protein expression.
HCE production automation makes it possible to offer this subcellular fraction of high and reproducible quality at very competitive prices.

Research Use
Our HeLa Cytoplasmic Extracts are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.

Also suitable for use in:

• purification and isolation of cytoplasmic protein;
• translatome analysis;
• circRNAs studies;
• ribosomal subunits studies;
• replication studies.

Downloads – Documents

References

• Angulo J, Ulryck N, Deforges J, Chamond N, Lopez-Lastra M, Masquida B, Sargueil B (2016) LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex. Nucleic Acids Res 44: 1309-1325
• Chamond N, Deforges J, Ulryck N, Sargueil B (2014) 40S recruitment in the absence of eIF4G/4A by EMCV IRES refines the model for translation initiation on the archetype of Type II IRESs. Nucleic Acids Res 42: 10373-10384
• King, Helen A., El-Sharif, Hazim F., Matia-González, Ana M., Iadevaia, Valentina, Fowotade, Adeola, Reddy, Subrayal M and Gerber, André P. (2017) Generation of ribosome imprinted polymers for sensitive detection of translational responses. Scientific Reports, 7 (1). ISSN 2045-2322
• Alexander R. Langley, Stefan Graf, James C. Smith, and Torsten Krude. (2016) Genome-wide identification and characterisation of human DNA replication origins by initiation site sequencing (ini-seq). Nucleic Acids Research, 2016 doi: 10.1093/nar/gkw760