Products – Apicell

HeLa Nuclear Extracts (Not dialysed)

Florent Rullière — Fri, 26 Apr 2019 09:12:46 +0000

HeLa cell
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.

Quality Control
Cultures are screened for the presence of bacteria, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.

HeLa Nuclear Extracts Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

The Nuclear Extracts are prepared according to:
Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-1489

Conc. Protein (Bradford) = +/- 6 mg/ml

No dialysis is performed during the preparation of this product. The preparation stopped after the ultra-centrifugation step. Buffer composition is then the same as the extraction buffer.

Research Use
Our HeLa Nuclear Extracts are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.

Source of HDAC activity, transcription factors, chromatin proteins and histones.

Also suitable for use in:

separation by SDS-PAGE;
Gel Shift assays used for protein-DNA interactions studies;
HDAC assays;
positive control for Western Blot assays;
in vitro splicing;
transcription factors studies;
cell division cycle and apoptosis studies;
spliceosome and other proteome studies (DNA ends, snRNP’s, DNA PKcs, …).

References

CV-1 Cytoplasmic Extracts

Florent Rullière — Fri, 02 Mar 2018 11:43:29 +0000

CV-1 cell line
This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
Derivation
The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies.

CV-1 Nuclei Production
CV-1 cells are grown in cell-factories under GLP conditions in our facility in Mons, Belgium.

Nuclei are prepared by low speed centrifugation, rinsed with phosphate buffer saline. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Cytoplasm is then snap frozen in liquid nitrogen and stored at -85°C.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).

CV-1 Nuclei

Florent Rullière — Fri, 02 Mar 2018 11:35:37 +0000

CV-1 Nuclei Production
CV-1 cells are grown in cell-factories under GLP conditions in our facility in Mons, Belgium.

Nuclei are prepared by low speed centrifugation, rinsed with phosphate buffer saline. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Nuclei are then snap frozen in liquid nitrogen and stored at -85°C.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).

CV-1 Cells Pellets

Florent Rullière — Mon, 19 Feb 2018 14:34:34 +0000

CV-1 cells Production
CV-1 cells are grown in cell-factories under GLP conditions in our facility in Mons, Belgium.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).

HeLa Nuclei

Florent Rullière — Thu, 13 Apr 2017 12:24:14 +0000

HeLa cell
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.

HeLa Nuclei Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Research Use
Our HeLa Nuclei are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.

Also suitable for use in:

entities production and purification of biological material from human origin used in many other applications (as core histones used in HAT assays);
Gel Shift and Western Blotting assay;
biochemical processing;
nucleic acid purification and research;
protein expression studies;
in vitro activity assays.

References

K562 Cell Pellets

Florent Rullière — Thu, 13 Apr 2017 12:21:43 +0000

K562 cell
The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.
The cell population has been characterized as highly undifferentiated and of the granulocytic series.
Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. The K-562 cell line has attained widespread use as a highly sensitive in vitro target for the natural killer assay.
See Pross, et al. for a detailed analysis of the in vitro assay of NK cells including the mathematics of quantitation of NK cell activity.
K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series.
The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. Cultures from the ATCC stock have been shown to exhibit this sensitivity for assessing human natural killer activity.
Karyological studies on various K-562 sublines have been classified into three groups (A,B,C) by Dimery, et al.
The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined.

K562 cells Production
K562 cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use Only
Our K562 cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Sera used in the culture medium are CE certified from South American origin.

HUT-78 Cell Pellets

Florent Rullière — Thu, 13 Apr 2017 12:18:18 +0000

HUT-78 cell
Derived from the peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2 and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for HUT-78.

HUT-78 cells Production
HUT-78 cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use Only
Our HUT-78 cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Sera used in the culture medium are CE certified from South American origin.

U937 Cell Pellets

Florent Rullière — Thu, 13 Apr 2017 12:18:04 +0000

U937 cell
U-937 cells are a model cell line used in biomedical research. They were isolated from the histiocytic lymphoma of a 37 year old male patient and are used to study the behaviour and differentiation of monocytes. U937 cells mature and differentiate in response to a number of soluble stimuli, adopting the morphology and characteristics of mature macrophages.

U937 cells Production
U937 cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use Only
Our U937 cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Sera used in the culture medium are CE certified from South American origin.

L363 Cell Pellets

Florent Rullière — Thu, 13 Apr 2017 12:14:56 +0000

L363 cell
Human plasma cell leukemia established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977; cells were described to be EBNA-negative and to express mRNA for proto-oncogene bcl-2 confirmed as human with IEF of AST, LDH, PEP B.

L363 cells Production
L363 cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use Only
Our L363 cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Sera used in the culture medium are CE certified from South American origin.

BV173 Cell Pellets

Florent Rullière — Thu, 13 Apr 2017 10:12:47 +0000

BV173 cell
The BV173 cell line was established from a B cell precursor leukemia.

BV173 cells Production
BV173 cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use Only
Our BV173 cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Sera used in the culture medium are CE certified from South American origin.