CV-1 Cytoplasmic Extracts


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CV-1 cell line
This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies.

CV-1 Nuclei Production
CV-1 cells are grown in cell-factories under GLP conditions in our facility in Mons, Belgium.

Nuclei are prepared by low speed centrifugation, rinsed with phosphate buffer saline. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Cytoplasm is then snap frozen in liquid nitrogen and stored at -85°C.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).

Additional information


1.0x10e8 cells, 2.5x10e8 cells, 5.0x10e8 cells


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